Publications

Fang D, Babich JM, Dangelmaier EA, Wall VA, Nachtergaele S. “A user guide to RT-based mapping of RNA modifications.” Methods in Enzymology, 705: 51-79 (2024).

Nachtergaele S. “Combating the myth of ‘innate genius’ in science.” Cell Chemical Biology 31: 376-379 (2023).

Gilbert WV and Nachtergaele S. “mRNA regulation by RNA modifications.” Annual Review of Biochemistry, 92: 175-198.

Nachtergaele S. “Enzymes flying under the radar: Cryptic METTL3 can persist in knockout cells.” PLOS Biology 20(7): e3001717 (2022). 

Draycott AS et al. “Transcriptome-wide mapping reveals a diverse dihydrouridine landscape including mRNA.” PLOS Biology 20(5): e3001622 (2022).  

Sepich-Poore C, et al. “The METTL5-TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation”. Journal of Biological Chemistry, 101590 (2022). 

Nachtergaele S and Krishnan Y. “Clinical Implications of Basic Research: New Vistas for Cell-Surface GlycoRNAs.” New England Journal of Medicine 385:658-660 (2021). 

Zhou H, Rauch S, Dai Q, Cui X, Zhang Z, Nachtergaele S, Sepich C, He C, Dickinson BC. “Evolution of a reverse transcriptase to map N¹-methyladenosine” Nature Methods 16: 1281-1288 (2019). 

Nachtergaele S and He C. “Chemical modifications in the life of an mRNA transcript” Annual Reviews of Genetics 52:23.1-23.24 (2018). 

Zhao BS, Nachtergaele S, Roundtree IA, He C. “Our views of dynamic N⁶-methyladenosine RNA methylation” RNA 24:268-272 (2018).

Huang H, Weng H, Sun W, Qin X, Shi H, Wu H, Zhao BS, Mesquita A, Liu C, Yuan CL, Hu Y, Huttelmaier S, Skibbe JR, Su R, Dong L, Sun M, Li C, Nachtergaele S, et al. “Recognition of RNA N⁶-methyladenosine by IGF2BP Proteins Enhances mRNA Stability” Nature Cell Biology, 20:285-295 (2018).

Su R*, Dong L*, Li C*, Nachtergaele S* et al. “R-2HG exhibits anti-tumor activity by targeting FTO/MYC signaling” Cell 172:90-105 (2018). *denotes equal contribution

Xiang Y*, Laurent B*, Hsu C*, Nachtergaele S et al. “m⁶A RNA methylation regulation regulates the UV-induced DNA damage response” Nature 543:546-573 (2017).

Li Z, Weng H, Su R, Weng X, Zuo Z, Li C, Huang H, Nachtergaele S et al. “FTO plays an oncogenic role in acute myeloid leukemia as a N⁶-methyladenosine RNA demethylase” Cancer Cell 31:127-141 (2016).

Nachtergaele S and He C. “The emerging biology of RNA post-transcriptional modifications” RNA Biol 14:156-163 (2016). 

Dominissini D*, Nachtergaele S*, Moshitch-Moshkovitz* S et al. “The dynamic N¹-methyladenosine methylome in eukaryotic messenger RNA.” Nature 530: 441-446 (2016). *denotes equal contribution. 

From previous work:

Kinnebrew M et al. “Patched 1 regulates Smoothened by controlling sterol binding to its extracellular cysteine-rich domain.” Science Advances 8: eabm5563 (2022).

Luchetti G, Sircar R, Kong JH, Nachtergaele S et al. “Cholesterol activates the G-protein coupled receptor Smoothened to promote morphogenetic signaling”. eLife, e20304 (2016).

Byrne EFX, Sircar R, Miller PS, Hedger G, Luchetti G, Nachtergaele S et al. “Structural basis of Smoothened regulation by its extracellular domains”. Nature 535:517-522 (2016).

Murada S, Navarro G, Truong A, Stewart DP, Arensdorf AM, Nachtergaele S et al. “Functional divergence in the role of N-linked glycosylation in Smoothened signaling”. PLoS Genet e1005473 (2015).

Montgomery SR, Nargizyan T, Meliton V, Nachtergaele S et al. “A novel osteogenic sterol compound for therapeutic development to promote bone growth: activation of Hedgehog signaling and osteogenesis through Smoothened binding.” J Bone Miner Res doi: 10.1002/jbmr.2213 (2014).\

Peyrot S*, Nachtergaele S*, Luchetti G, Mydock-McCrane LK et al. “Tracking the subcellular fate of an oxysterol with click chemistry reveals a transport pathway to the Golgi.” J Biol Chem 289:11095-11110 (2014). *denotes equal contribution.

Nachtergaele S*, Whalen DM*, Mydock LK, Zhao Z et al. “Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.” eLife e01340 (2013). *denotes equal contribution.

Nachtergaele S,  Mydock LK, Krishnan K, Rammohan J et al. “Oxysterols are allosteric activators of the oncoprotein Smoothened.” Nature Chemical Biology 8: 211-220 (2012).

Bodnarchuk M, Li L, Fok A, Nachtergaele S et al. “Three-Dimensional Nano-crystal Superlattices Grown in Nanoliter Microfluidic Plugs.” J Am Chem Soc 133: 8956-8960 (2011).

Li L, Nachtergaele S, Seddon AM, Tereshko V et al. ”Simple Host-Guest Chemistry To Modulate the Process of Concentration and Crystallization of Membrane Proteins by Detergent Capture in a Microfluidic Device.” J Am Chem Soc 130: 14324-14328 (2008).